Composite

Part:BBa_M36789:Design

Designed by: Marisa Borja & Allison Mateo   Group: Stanford BIOE44 - S11   (2012-12-07)

[apFAB46][apFAB687BCD9][NarL][apFAB391][BBa_K216005]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was the ideal sequence, but synthesis issues led to two mutants.

The first mutant had the following mutations: 864 (substitution T --> C in the terminator), 22 (deletion T in the constitutive promoter), 23 (deletion C in the constitutive promoter)

The second mutant had the following mutations: 131 (deletion T in the RBS), 136 (deletion T in the NarL gene), 137 (deletion C in the NarL gene)

Source

The NarL gene comes from the E. coli genome and was found on the UniProt database. The PyeaR promoter also comes from the E. coli genome and was found on the Parts Registry, designed by the 2009 Edinburgh iGEM team.

References

Darwin, A., Ziegelhoffer, E., Kiley, P., Stewart, V. 1998. Fnr, NarP, and NarL Regulation of Escherichia coli K-12 napF (Periplasmic Nitrate Reductase) Operon Transcription In Vitro. Journal of Bacteriology, p. 4192-4198