Coding

Part:BBa_M36770:Design

Designed by: Preston Lim   Group: Stanford BIOE44 - S11   (2014-10-23)


ClpP Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure to screen for BamHI and BbsI restriction sites, and palindromic sequences of above 10 bp.


Source

EcoCyc: http://biocyc.org/ECOLI/sequence?object=EG10158

References