Part:BBa_M36667:Experience

Having confirmed the transformation, optimal glutathione concentration (0.1 mM), standard β-gal baseline under aerated conditions, and the negative correlation between N2 bubbling time and dissolved oxygen concentration, we examined the effect of various concentrations of dissolved oxygen concentrations on β-gal presence in E. coli. As the concentration of dissolved oxygen decreases, the normalized A420 increases albeit at very low levels. Assuming a linear relationship, the best fit trend-line indicates that as oxygen concentration decreases there is a mild increase in β-gal presence at 0.897 miller units per minute of bubbling time(R2=0.2456). This result however proves insignificant owing to the low correlation value. However, calculating the best fit, max slope, and min-slope trend-line of the measured dissolved oxygen concentration versus the normalized A420 yields a negative correlation between dissolved oxygen concentration and normalized A420. Assuming a uniformly probability distribution of slopes between the maximum and minimum slopes for a correlation between the two variables, taking the difference between the max and minimum slopes and dividing it by the minimum slope (negative slope) indicates that there is a 0.67 probability of a negative correlation between dissolved oxygen concentration and normalized A420. Albeit insignificant, our results still demonstrate to an extent the presence of a relationship between the dissolved oxygen concentration and the presence of β-gal in E. coli. Examining the kinetic assay, as time increases the normalized A420 increases with decreasing oxygen concentration for all time points up until 4 minutes into the kinetic assay (Supplemental Materials Appendix E Table 1). From that time point onward we observed an equalizing A420 over all oxygen concentrations. From these results, we can only conclude that there is only the possibility of a negative correlation between β-gal and dissolved oxygen concentration. It is entirely possible that there could be no correlation between the two variables but given the data, there stands a slim possibility of a correlation.

Applications of BBa_M36667

Plasmid Name: hypoxia FNR/pepT (hfp)

DNA 2.0 Gene#: 151285

Organism: E. Coli

Device Type: Sensor

Box Name: BIOE44 S14

Barcode #s:

0133011370

0133009717

0133009709

0133010300

0133009668

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