Measurement

Part:BBa_M36665

Designed by: Gordon Sun   Group: Stanford BIOE44 - S11   (2014-05-06)


Hypoxia Sensor

Part utilizes FNR transcription factor with E. Coli constitutive promoter, which regulates pepT promoter downstream with Gemini actuator (with only LacZ gene).

FNR regulated by O2, inactive when exposed to O2; requires Glutathione and cysteine desulfurase to reactivate, of which cysteine desulfurase is already present within E. Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None