Signalling
Part:BBa_M36663:Design
Designed by: Frankie Willcox Group: Stanford BIOE44 - S11 (2013-10-25)
Design notes: We wanted to use dimerized fur protein to repress downstream expression of the actuator, so we put our protein binding site in between the -10 and -35 box to allow for the protein to bind and inhibit transcription. Sequence source: Lavrrar, Jennifer and Mark McIntosh. “Architecture of Fur Binding Site: a Comparative Analysis”. Journal of Bacterial Biology.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC151488/. Web.