Composite

Part:BBa_M36558:Design

Designed by: Ashley Henderson   Group: Stanford BIOE44 - S11   (2014-10-24)


PoPS estrogen receptor beta and ERE operon.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 84
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1734
    Illegal NheI site found at 1757
    Illegal PstI site found at 1228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 84
    Illegal BglII site found at 1178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 84
    Illegal PstI site found at 1228
    Illegal NgoMIV site found at 204
    Illegal AgeI site found at 1576
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design we ordered the basic parts into a precise order that would allow the ER beta to be made and able to arrest the transcription of the the gene that follows by binding to the ERE operon.


Source

The parts that make up this composite are as follows and in this order: BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562 They come from both the parts registry and are edited basic parts that we manually added to the registry.

References