Composite

Part:BBa_M36552:Design

Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong   Group: Stanford BIOE44 - S11   (2015-10-22)


OYE1 Composite Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
    Illegal NgoMIV site found at 218
    Illegal NgoMIV site found at 266
    Illegal NgoMIV site found at 272
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design was done using a DNA2.0 software and GeneDesigner2.0 quality checks. The recommended design (in terms of promoters, RBS, tags, and terminators) was taken without amendments.


Source

All of these are basic parts that have each of their sources documented individually.

References