Coding

Part:BBa_M36550:Design

Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong   Group: Stanford BIOE44 - S11   (2015-10-22)


OYE1 Protein Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 829
    Illegal PstI site found at 550
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 829
    Illegal PstI site found at 550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 829
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 829
    Illegal PstI site found at 550
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 829
    Illegal PstI site found at 550
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 145
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Again, this came directly from GenBank (http://www.ncbi.nlm.nih.gov/nuccore/JQ765594.1) with the caveat of having its final codon (i.e., its stop codon) cut out.


Source

This part comes directly from GenBank (http://www.ncbi.nlm.nih.gov/nuccore/JQ765594.1) except has its final codon (i.e., the stop codon) cut out. Note that this enables the sequence to be used using a FLAG/HIS tag afterwards.

Final Codon (i.e., Stop Codon): Removed

References