Regulatory

Part:BBa_M36543:Design

Designed by: Anna McGregor, Maya Anjur-Dietrich, and Kay Hung   Group: Stanford BIOE44 - S11   (2012-12-07)

Calcium Induced Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1732


Design Notes

There were no changes to the original sequence, even for optimization purposes or to minimize repeats, due to the lack of information about mammalian promoters. This meant that any codon optimization could affect an area critical to the function of the promoter.

Input: Ca 2+ Output: PoPS

Source

The part comes from the 2000 base pairs upstream of the human prolactin (PRL) gene.

References

1) White, B., Power, E., & Fay, F. (1989). Calcium regulation of prolactin gene expression: Opposing effects of extracellular cacl2 and ca2 ionophores. Molecular endocrinology, 3(11), 1757-1764. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2514348

2) Preston, G. M., Billis, W. M., & White, B. A. (1990). Transcriptional and posttranscriptional regulation of the rat prolactin gene by calcium. Molecular cell biology, 10(2), 442–448. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC360809/