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Part:BBa_M36528:Design

Designed by: Valerie Wesley Scott   Group: Stanford BIOE44 - S11   (2015-10-22)


In the presence of ATC, produces lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensured codons were optimized for yeast. Short random (10 bp) region RBS and the actual gene put in. Eliminated restriction enzyme sites (Bbs1, Bsa1,BsmB1).


Source

LacI Gene: Part:BBa_K642001:Design (parts.igem.org)

References