
Part:BBa_M36525:Design
LacI
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Even though this LacI gene is adapted from a yeast optimized version, we decided that since the LacI gene is typically from bacteria, it would be best to run it through a yeast optimizer to double check that it is certainly optimized. To do this, we simply ran the LacI gene from the BBa_K642001 part through the IDT Codon Optimization application. In addition, we added a stop codon (TAA) to the end of the original BBa_K642001 part.
This part was inserted into DNA2.0 Yeast Expression Vector pD1224 (see references).
Source
A selected portion of the BBa_K642001 part on iGem's Registry of Standard Biological Parts.
References
IDT Codon Optimization https://www.idtdna.com/CodonOpt
Yeast Expression Vector pD1224 https://www.dna20.com/eCommerce/catalog/datasheet/197