DNA

Part:BBa_M36430:Design

Designed by: Anna Jaffe   Group: Stanford BIOE44 - S11   (2014-10-23)

Bxb1 attP - reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 22


Design Notes

This sequence is simply the Bxb1-attP site in the 5' to 3' direction (to create the flipping outcome) as opposed to the 3' to 5' direction (which results in the excision outcome).

Source

This site was obtained from the supplemental information of the cited paper (and is in the same orientation that it appears in their construct).

References

Bonnet J, Subsoontorn P, Endy D. 2014. Rewritable Digital Data Storage In Live Cells Via Engineered Control Of Recombination Directionality. PNAS. 109(23): 8884-8889.

BBa_I20290