Part:BBa_M36362:Design
DbjA Haloalkane Dehalogenase
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 379
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 379
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 379
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 379
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 379
Illegal NgoMIV site found at 895
Illegal AgeI site found at 624 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 685
Design Notes
The amino acid sequence was backtranslated through Gene Designer's genetic algorithm (Maximum Generations: 100; Maximum Mutation Rate: 0.05; Minimum Best Individual Selection Rate: 0.9; Population Size: 50; Number of Progeny: 25) into a codon set optimized for Escherichia coli K12. This algorithm was given additional parameters to avoid BbsI, BsmBI, and BsaI restriction sites (at a weight of 1), hairpins (at a weight of 1), and repeats of at least 10 bp (at a weight of 1). A TAA stop codon was then added at the end of the resulting DNA sequence.
Source
This part was sourced from the genomic sequence of the soil bacterium Bradyrhizobium japonicum USDA110, at Accession NP_767727 and Gene ID 27376198. No mutations were made.