RBS

Part:BBa_M36361:Design

Designed by: Ethan Li   Group: Stanford BIOE44 - S11   (2013-10-25)

5' Bicistronic UTR (apFAB866, GENE10_BCD2), No ATG Start


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was copied, and the ATG start codon was removed.


Source

This part was sourced from the Supplemental Table 1 of Mutalik, et al., where it was constructed as a candidate sequence-independent BCD.

References