Composite

Part:BBa_M36242:Design

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)


Mature EP-B2 YepF Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 414
    Illegal BglII site found at 1185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 853
    Illegal AgeI site found at 937
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1604


Design Notes

Testing whether mature EP-B2 protein is folded properly and is then successfully secreted versus YebF.

Source

Mature EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0

References