Coding

Part:BBa_M36188:Design

Designed by: Aaron Thayer   Group: Stanford BIOE44 - S11   (2012-05-03)


Ligninase Coding Region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This enzyme works at pH 4.5 as found here: http://www.springerlink.com/content/7616k56005852420/fulltext.pdf?MUD=MP

Sanroman et al. "Optimum stability conditions of pH and temperature for ligninaseand manganese-dependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP." World Journal of Microbiology and Biotechnology. 22: 607-612. 2006.


Source

MAFKQLFAAISLALLLSAANAAAVIQKRATCSNGKYVGDASCCAWFDVLDDIQNLFHGGQCGAEAHESIRLVFHDSIAISPAMEAQGKFGGGGADGSIMIFDDIETAFHPNIGLDEIVKLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHTVDQIINRVNDAGEFDELELVWMLSAHSVAAVNDVNPTVQGLPFDSTPGIFDSQFFVETQLRGTAFPGSGGNQGEVESPLPGEIRIQSDHTIARDSRTACEWQSFVNNQSKLVDDFQFIFLALTQLGQDPNAMTDCSDVIPQSKPIPGNLPFSFFPAGKTIKDVEQACAETPFPTLTTLPGPETSVQRIPPPPGA

References