Coding
Part:BBa_M36160:Design
Designed by: Eric Kofman, Matt Kandath Group: Stanford BIOE44 - S11 (2012-06-04)
T7 17.5 protein (holin)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note: this sequence begins with a GTG start codon when expressed naturally. When designing for E. coli, changing the start codon to ATG is suggested for optimization purposes.
Source
http://www.ncbi.nlm.nih.gov/nuccore/NC_001604.1?report=genbank&from=36344&to=36547