Composite
Part:BBa_M36154:Design
Designed by: Monica Chin Group: Stanford BIOE44 - S11 (2014-10-23)
NifA sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 499
Illegal PstI site found at 649 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 499
Illegal PstI site found at 649 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1539
Illegal BglII site found at 2003 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 499
Illegal PstI site found at 649 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 499
Illegal PstI site found at 649
Illegal NgoMIV site found at 921
Illegal NgoMIV site found at 2044
Illegal AgeI site found at 1801 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In designing this part, we estimated the locations of the promoter sequences within the nif cluster. This may not be entirely accurate and may include extraneous sequences.
Source
The sequences in this part come from the genome of Azotobacter vinelandii.