Part:BBa_M36148:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_M36148

Insertion into Saccharomyces cerevisiae through either pD1201 or pD1231, found on DNA 2.0. This part has successfully transformed into S. cerevisiae using pD1201.

Stanford Location Plasmid Name = AlgA DNA 2.0 Gene # = pD1201 Organism = Saccharomyces cerevisiae Device Type = Actuator Barcode # of Glycerol Stock = 0133011781, 0133011367, 0133027021, 0133024492 Box Label = BioE44 F15 Box 2

User Reviews

UNIQ81acb42fab174e01-partinfo-00000000-QINU Chloe Rickards, Review #1

A team of three individuals used this part in a classroom lab space, in BIOE 44 at Stanford University. We failed to detect the production of alginate A with our part. However, more tests need to be done in order to definitively conclude this part as non-functional. We inserted this gene into a plasmid with a galactose inducible promotor, but failed to introduce galactose into our media until later in the experiment. As a result, we could not detect AlgA through Western blotting. We expect better results either by introducing galactose into the system earlier, or by using a strong constitutive promoter. Additionally, we experienced difficulties measuring fructose-6-phosphate consumption using our BioVision PicoProbe fructose-6-phosphate assay. Future experimenters should either choose a different method through which to measure fructose-6-phosphate consumption, or find a way to measure mannose-6-phosphate directly. UNIQ81acb42fab174e01-partinfo-00000001-QINU