Regulatory

Part:BBa_M36146:Design

Designed by: Jane Hae Soo Shin   Group: Stanford BIOE44 - S11   (2012-06-06)


Cold-inducible promoter for HeLa cells


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the promoter was found in CHO cells first, we used BLAST site to find a similar genetic sequence in HeLa cells.


Source

The sequence has been BLASTed to HeLa cells because it was originally found in CHO cells. http://ukpmc.ac.uk/articles/PMC3118111/pdf/1472-6750-11-51.pdf

References