Protein_Domain
Part:BBa_M36142:Design
Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi Group: Stanford BIOE44 - S11 (2015-10-22)
Mature Cysteine Proteinase EP-B2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 381
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 49
Illegal AgeI site found at 133
Illegal AgeI site found at 472 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mature version of EP-B2 protein found in barley that breaks down gliadin found in gluten. Testing whether mature EP-B2 is enough for cells to survive.
Source
DNA sequence for mature EP-B2 generated from amino acid sequence in "Computational Design of an α-Gliadin Peptidase".