Part:BBa_M36111:Design

Lysis gene E for phiX174, synonymous mutation on gene J's rbs inside E

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

When mutating base pairs, first priority was mutating into a c or t, second was maintaining a similar codon usage as the original.

Source

wild type phiX174 gene E.

References

Van der Avoort HG, Teertstra R, Versteeg R, Weisbeek PJ. Genes and regulatory sequences of bacteriophage phi X174. Biochim Biophys Acta. 1983 Oct 13;741(1):94–102.

F. Sanger, A. R. Coulson, T. Friedmann, G. M. Air, B. G. Barrell, N. L. Brown, J. C. Fiddes, C. A. Hutchison III, P. M. Slocombe, M. Smith, The nucleotide sequence of bacteriophage [phi]X174, Journal of Molecular Biology, Volume 125, Issue 2, 25 October 1978, Pages 225-246, ISSN 0022-2836, DOI: 10.1016/0022-2836(78)90346-7. (http://www.sciencedirect.com/science/article/pii/0022283678903467)

Grzegorz Kudla, Andrew W. Murray, David Tollervey, and Joshua B. Plotkin. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science 10 April 2009: 324 (5924), 255-258. [DOI:10.1126/science.1170160]

Calendar, Richard. "φX174 Et Al., the Microviridae." The Bacteriophages. New York: Plenum Pub., 1988. Print.

Fane, Bentley A., Karie L. Brentlinger, April D. Burch, Min Chen, Susan Hafenstein, Erica Moore, Christopher R. Novak, and Asako Uchiyama. "TheBacteriophages.org."TheBacteriophages.org. 2006. Web. 07 June 2011. <http://www.thebacteriophages.org/frames_0110.htm>.

phiX174 genome - Refseq: NC_001422 - http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=10126