Plasmid_Backbone

Part:BBa_M36098:Design

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-25)


E. coli actuator test rig


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal NheI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal BglII site found at 1315
    Illegal BamHI site found at 2053
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
    Illegal AgeI site found at 1583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2330


Design Notes

DNA2.0 licenses this plasmid from Xbrane and charges for its use.


Source

Xbrane is has a patent on this plasmid, Patent Number: US008138324.

References

US Patent Number: US008138324

Biotechniques. 2006 Mar;40(3):355-64. Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, Surber MW.