Plasmid_Backbone

Part:BBa_M36098

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-25)


E. coli actuator test rig

E. coli test vector for actuators. Has a rhamnose inducible, glucose repressible promoter. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0. Vector is licensed to DNA2.0 by Xbrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal NheI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal BglII site found at 1315
    Illegal BamHI site found at 2053
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1995
    Illegal EcoRI site found at 2098
    Illegal XbaI site found at 2047
    Illegal AgeI site found at 1583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2330


[edit]
Categories
//plasmidbackbone
Parameters
None