Composite
SoDA

Part:BBa_M36072:Design

Designed by: Justin Diep, Alex Rosay, Ben Blankenmeister   Group: Stanford BIOE44 - S11   (2012-12-07)


Superoxide Dismutase A Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 440
    Illegal PstI site found at 377
    Illegal PstI site found at 687
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 440
    Illegal PstI site found at 377
    Illegal PstI site found at 687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 440
    Illegal XhoI site found at 137
    Illegal XhoI site found at 221
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 440
    Illegal PstI site found at 377
    Illegal PstI site found at 687
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 440
    Illegal PstI site found at 377
    Illegal PstI site found at 687
    Illegal NgoMIV site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The encoded enzyme sequence (BBa_M36070) was derived from converting the amino acid sequence of sodA obtained from Durfee et al. from the University of Wisconsin (obtained from E. coli strain: k-12, substrain: DH10B genome).


Source

Durfee et al. "The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse" 1 Feb 2008.

References