Coding

Part:BBa_M36060:Design

Designed by: Taylor Marie Chavez   Group: Stanford BIOE44 - S11   (2015-10-23)


Poly-beta-hydroxybutyrate Growth Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design considerations include keeping the DNA sequence less than 1500 bp, avoiding the sites BsbI, BsaI, and BsmBI, and having less than 5 repeats of 10 bp.


Source

Source of the part is the genomic sequence Xanthomonas campestris pv. raphani 756C, specifically G0CFI5.

References