Device

Part:BBa_M36053:Design

Designed by: Ryan Kent   Group: Stanford BIOE44 - S11   (2011-05-02)

DNA damage sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 782
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 782
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 782
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct is using a medium strength promoter and 5'UTR so that the amount of lexA won't be so great as to not allow for endogenous levels of recA


Source

composite

References

Analysis of Escherichia coli RecA Interactions with LexA, CI, and UmuD by Site-Directed Mutagenesis of recA JULIE A. MUSTARD1 and JOHN W. LITTLE

Control of recA Gene RNA in E. coli: Regulatory and Signal Genes Ann McPartland, Linda Green and Harrison Echo

DNA binding properties of the LexA repressor M Schnarr, P Oertel-Buchheit, M Kazmaier, M Granger-Schnarr

Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria Nir Friedman1, Shuki Vardi1[, Michal Ronen, Uri Alon, Joel Stavans

The bacterial LexA transcriptional repressor M. Butalaa, D. Zgur-Bertokb and S. J. W. Busbya

Assigning numbers to the arrows: Parameterizing a gene regulation network by using accurate expression kinetics Michal Ronen, Revital Rosenberg, Boris I. Shraiman, and Uri Alon

Structure of the LexA–DNA complex and implications for SOS box measurement Adrianna P. P. Zhang, Ying Z. Pigli & Phoebe A. Rice