Part:BBa_M36042:Design
EfeU elemental iron (II) transporter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Standard K-12 E. coli strains have a frame-shift mutation which makes this gene inactive. The given sequence was conceptually reconstructed from the frame-shifted version using the wt allele from Shigella sonnei Ss046 as a guide (see Ecogene database). The Ecogene entry has an "N" for the nucleotide which is uncertain, but a curator comment indicates that the correct residue is likely glycine. Thus we chose N = G in the final sequence.
From the original paper: the ycdN gene is part of the ycdNOB tricistronic operon which is expressed in response to iron deprivation in a Fur-dependent manner. It is an integral cytoplasmic membrane protein exhibiting seven transmembrane helices. It was shown to improve growth in E. coli lacking all other known iron uptake systems, and functioned as ferrous iron permease in proteoliposomes in vitro. The REXXE motifs are implicated in iron translocation in other members of the OFeT protein family.
Source
This gene was originally studied in wild-type Nissle 1917 E. coli. The sequence came from the Ecogene entry EG13860. K-12 Gene Accession Number: ECK1007. MG1655 Gene Identifier: b4490 b1017 b1016.
References
Grosse C, Scherer J, Koch D, Otto M, Taudte N, et al. A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Mol Microbiol. 2006;62:120–131. PMID: 16987175.