Part:BBa_M36038:Design
PoPS->Alpha Factor Mating Pheromone
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 30
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 30
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 30
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 30
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part is designed to be put directly after a Rhamnose sensor in a plasmid containing an ampicillin resistance gene in E. coli.
Source
[parts.igem.org]
Sequence of the Alpha Factor Mating Pheromone Generator from Yeast Genome
References
1. Severin FF, and Hyman AA. Pheromone induces programmed cell death in S. cerevisiae. Curr. Biol 2002. 12:R233-235.
2. Pozniakovsky AI, Knorre DA, Markova OV, Hyman AA, Skulachev VP, Severin FF. Role of mitochondria in the pheromone- and amiodarone-induced programmed death yeast. J Cell Biol 2005; 168: 257-269.
3. Carmona-Gutierrez D, Eisenberg T, Büttner S, Meisinger C, Kroemer G and Madeo F. Apoptosis in yeast: triggers, pathways, subroutines. Cell Death and Differentiation 2010; 17: 763–773.
4. Hoffman G, Garrison TR, and Dohlman HG. Analysis of RGS proteins in Saccharomyces cerevisiae, Methods Enzymol. 2002. 344: 617-631