Composite

Part:BBa_M36038:Design

Designed by: Joe Schlicht, Trevor! Kalkus, and David Eduardo Arrambide Montemayor   Group: Stanford BIOE44 - S11   (2011-12-20)

PoPS->Alpha Factor Mating Pheromone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part is designed to be put directly after a Rhamnose sensor in a plasmid containing an ampicillin resistance gene in E. coli.


Source

[parts.igem.org]

Sequence of the Alpha Factor Mating Pheromone Generator from Yeast Genome

References

1. Severin FF, and Hyman AA. Pheromone induces programmed cell death in S. cerevisiae. Curr. Biol 2002. 12:R233-235.

2. Pozniakovsky AI, Knorre DA, Markova OV, Hyman AA, Skulachev VP, Severin FF. Role of mitochondria in the pheromone- and amiodarone-induced programmed death yeast. J Cell Biol 2005; 168: 257-269.

3. Carmona-Gutierrez D, Eisenberg T, Büttner S, Meisinger C, Kroemer G and Madeo F. Apoptosis in yeast: triggers, pathways, subroutines. Cell Death and Differentiation 2010; 17: 763–773.

4. Hoffman G, Garrison TR, and Dohlman HG. Analysis of RGS proteins in Saccharomyces cerevisiae, Methods Enzymol. 2002. 344: 617-631