Coding

Part:BBa_M36027

Designed by: Jon Rodriguez   Group: Stanford BIOE44 - S11   (2011-05-03)

phiX174 gene C - mutated rbs and start codon of D

Modified version of the PhiX174 gene that causes the switch from stage II replication (copying of the dsDNA phagemid using the host cell's machinery) to stage III replication (rolling circle packaging of one strand of a circle into the capsid). Took the wt gene C from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene D.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 30
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None