Coding
NarL
Part:BBa_M33030:Design
Designed by: Scott Runyon Group: Stanford BIOE44 - S010 (2010-06-02)
NarL: Regulates nitrate reduction (strong RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
Illegal BglII site found at 398 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Initial NarL part was ordered with BBa sites but without promoter or RBS. Primers were ordered separately that added these and of course a new set of identical restriction sites, the original ones removed.
Source
Coding sequence taken from NCBI: http://www.ncbi.nlm.nih.gov/gene/945795 and synthesized in Palo Alto by DNA 2.0