Project

Part:BBa_M31516:Design

Designed by: Han Zhu   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-26)


Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2220
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to refactor the genome of the M13 to faciliate future engineering. In doing so, we want to "clean up" the wildtype genes so that they are independent of each other and can be easily engineered independent of each other. We also want to delete unnecessary or obscure sequences for better human understanding of the M13 genome.


Source

Sequence taken from the copy of the M13K07 genome available at: http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt

References