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Coding
Part:BBa_M31510:Design
Designed by: Kate Broadbent Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07 (2007-02-24)
Changes: Where and Why
- bp 307-312: want to remove Gene 5 (-10) promoter binding sequence
- frame = TTG ACA : Leu Thr
- change to = CTG ACC : Leu Thr
- frame = TTG ACA : Leu Thr
New 0-cutter site
- Acc651 at end