Coding

Part:BBa_M31502:Design

Designed by: Kate Broadbent   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-24)

Changes: Where and Why

  1. bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
    • frame = TTG ACA : Leu Thr
      • change to = CTG ACC : Leu Thr
  2. Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome

New 0-cutter site

  1. AatII at end