Coding
Part:BBa_M31281:Design
Designed by: Robert Warden Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07 (2007-02-27)
- Started with wt Gene VIII
- Designed Elements
- Codon Optimized for E. Coli
- FspI Site added into start codon
- Allows easy addition to pVIII N-Terminus
- Needed to mutate Lys2 to Arg2 which have similar chemical properties