Composite

Part:BBa_M31107:Design

Designed by: Michael Lee   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2008-05-01)


Produces Beta-carotene from the biochemical input GGPP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contact biomagik@mit.edu for specific questions.


Source

Each gene is taken from a different bacterial organism: CrtB-R capsulatus CrtI-S solfataricus CrtY-C pelagibacter

Our sequence has been codon optimized for the Lactobacillus acidophilus strain NCFM.

The promoter, RBS and terminator were taken from the Registry website.

References