Composite

Part:BBa_M1988:Design

Designed by: David Harris   Group: UtahState_BE5930_-_S11   (2012-03-28)


Composite with rpsT gene revised


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 185
    Illegal PstI site found at 375
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 171
    Illegal NheI site found at 361
    Illegal PstI site found at 375
    Illegal NotI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 171
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 185
    Illegal PstI site found at 375
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 171
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 185
    Illegal PstI site found at 375
    Illegal NgoMIV site found at 1515
    Illegal NgoMIV site found at 2778
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4306
    Illegal SapI site found at 3223


Design Notes

I found these parts on the registry


Source

the source is from methylobacterium chloromethanicum.

References