Composite
Part:BBa_M1542:Design
Designed by: Brian Smith Group: UtahState_BE5930_-_S11 (2012-04-22)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4472
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2139
Illegal BglII site found at 2802
Illegal BamHI site found at 4991
Illegal XhoI site found at 4892 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1069
Illegal AgeI site found at 4308
Illegal AgeI site found at 4365 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
See subparts.
Source
See subparts.