Coding

Part:BBa_M1525:Design

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-10)

Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codons were optimized to be used in E. coli, and any restriction enzyme sites were removed. An 8-His tag was added to the end of the sequence.

Source

This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).

References