DNA

Part:BBa_M12204:Design

Designed by: Grant Robinson   Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10   (2011-01-07)

Amino Acids 207-240 of E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 26


Design Notes

Not applicable. Forward primer: gtttcttcgaattcgcggccgcttctagagctttcgaaagatcccaacgaaaagagag Forward primer: gtttcttcgaattcgcggccgcttctagagctttcgaaagatcccaacgaaaagagag Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

M12204f: gtttcttcgaattcgcggccgcttctagagctttcgaaagatcccaacgaaaagagag

M12204r: tttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

Source

BBa_E0040.

References