Part:BBa_M12068:Design
BioBrick-Compatible Broad-Host-Range Vector pPMQAK1
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1924
Illegal suffix found in sequence at 2621 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1924
Illegal SpeI site found at 2622
Illegal PstI site found at 2636
Illegal NotI site found at 1930
Illegal NotI site found at 2629 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1924
Illegal BamHI site found at 2609
Illegal XhoI site found at 817 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1924
Illegal suffix found in sequence at 2622 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1924
Illegal XbaI site found at 1939
Illegal SpeI site found at 2622
Illegal PstI site found at 2636
Illegal AgeI site found at 3681
Illegal AgeI site found at 4082 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 963
Illegal BsaI.rc site found at 2059
Design Notes
According to the authors' article, although BioBrick expression in non-Escherichia coli organisms was indeed possible with the use of the vector, there are issues with promoter expression (in particular, Plac and Ptet are essentially dysfunctional) and apparent issues with RNA Polymerase interactions with at least some protein-mediated activation/inhibition reactions; in particular, one designed promoter, Ptrc2O was properly repressed by LacI but was unable to return to constitutive activity with the addition of surplus IPTG. In short, protein expression via the pPMQAK1 shuttle vector is apparently viable, but dedicated promoters may well have to be designed depending on the chassis.
Source
http://www.ncbi.nlm.nih.gov/pubmed/20236988 [Location of the original article containing reference to pPMQAK1.]
http://www.ncbi.nlm.nih.gov/nuccore/292601742 [Location of the pPMQAK1 vector expressed here in GenBank.]