Part:BBa_M11421:Design

Streptomyces Shuttle Vetor Thiostrepton Resistance

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 11868
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2075
Illegal NotI site found at 2201
Illegal NotI site found at 2294
Illegal NotI site found at 3386
Illegal NotI site found at 3415
Illegal NotI site found at 4123
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 11868
Illegal XhoI site found at 1559
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 11868
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 11868
Plasmid lacks a suffix.
Illegal XbaI site found at 11883
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 719
Illegal NgoMIV site found at 1513
Illegal NgoMIV site found at 2851
Illegal NgoMIV site found at 3630
Illegal NgoMIV site found at 4000
Illegal NgoMIV site found at 5732
Illegal NgoMIV site found at 5789
Illegal AgeI site found at 603
Illegal AgeI site found at 1794
Illegal AgeI site found at 6577
Illegal AgeI site found at 7386
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 4594
Illegal BsaI site found at 5422
Illegal BsaI.rc site found at 1503
Illegal BsaI.rc site found at 5082
Illegal BsaI.rc site found at 6380
Illegal BsaI.rc site found at 10933
Illegal SapI.rc site found at 241

This is a shuttle vector for Streptomyces.

This originated from the plasmid pRM5. Site-directed mutagenesis was used to remove specific restriction sites to make it biobrick compatible.