Coding
Part:BBa_M11106:Design
Designed by: Charles Miller Group: UtahState BE5930 - S09 UtahState BE5930 - S10 (2010-04-01)
example
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 61
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 61
Illegal NheI site found at 7
Illegal NheI site found at 34
Illegal NheI site found at 74
Illegal NheI site found at 98
Illegal NheI site found at 105
Illegal NheI site found at 109
Illegal NheI site found at 130 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 61
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 61
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 61
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pstI sites needed to be mutated
Source
genomic DNA DNA synthesis