Coding

Part:BBa_M11080:Design

Designed by: logan christenson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-16)

silE (coding sequence for Silver binding protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 458
    Illegal AgeI site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PstI Restriction Enzyme site at base pair 458-463 will need to be mutated if standard biobrick assembly is used.

Source

Joint Genome Institute Gene Object ID 640940792 Chromosome: plasmid pAPEC-O1-R Escherichia coli APEC O1 plasmid pAPEC-O1-R: NC_009838 (241387bp) DNA coordinates: 120874..121422 (-)(549bp)


References

U.S. Department of Energy Joint Genome Institute Gene object ID: 640940792 http://img.jgi.doe.gov/cgi-bin/pub/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=640940792