Coding

Part:BBa_M11076:Design

Designed by: Elisabeth Linton   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-16)

NrtA: nitrate/nitrite binding protein coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 878
    Illegal BamHI site found at 92
    Illegal BamHI site found at 313
    Illegal BamHI site found at 540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal NgoMIV site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no restriction enzymes sites within the sequence that need to be mutated for standard Biobrick assembly.

Source

Gene Object ID: 637872195, Genome: Synechococcus sp. JA-3-3Ab, DNA Coordinates: 621322..622644

References

U.S. Department of Energy Joint Genome Institute Gene object ID: 637872195 http://img.jgi.doe.gov/cgi-bin/pub/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=637872195