Coding

Part:BBa_M11063:Design

Designed by: Dan Nelson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-14)


pfaE omega-3 polyunsaturated fatty acid synthase (mutated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the original sequence there were EcoR1 and Pst1 sites at 436, and 126 bp resepectivly. The mutations were made and the sequence is provided.


Source

Moritella marina strain MP-1.

References