Part:BBa_M11058:Design

pfaA gene for omega-3 fatty acid synthesis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1255
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4071
  Illegal BglII site found at 4464
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2856
  Illegal NgoMIV site found at 3927
  Illegal NgoMIV site found at 4215
  Illegal NgoMIV site found at 4521
  Illegal NgoMIV site found at 4830
  Illegal NgoMIV site found at 5268
  Illegal NgoMIV site found at 6177
  Illegal AgeI site found at 1102
  Illegal AgeI site found at 2947
  Illegal AgeI site found at 4239
  Illegal AgeI site found at 4639
  Illegal AgeI site found at 4875
  Illegal AgeI site found at 6249
  Illegal AgeI site found at 6385
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2934
  

Design Notes

This sequence was mutated to remove a SpeI, XbaI, Pst1 restriction sites at 2337 bp, 4050 bp, and 3051 bp respectively

Source

Photobacterium profundum strain SS9