Coding

Part:BBa_M11032:Design

Designed by: Erik Ostler   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-16)

Cellobiohydrolase [Thermobifida fusca YX]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 419
    Illegal PstI site found at 690
    Illegal PstI site found at 711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 419
    Illegal PstI site found at 690
    Illegal PstI site found at 711
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 419
    Illegal BamHI site found at 511
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 419
    Illegal PstI site found at 690
    Illegal PstI site found at 711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 419
    Illegal PstI site found at 690
    Illegal PstI site found at 711
    Illegal NgoMIV site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was imported into DNA 2.0 where the nucleic acid sequence most appropriate for E. coli was determined. Two stop codons were then placed at the end of the sequence, and a OmpA secretion signal sequence was added to the beggining.

Source

Amino acid sequence was found in the NCBI database.

References

http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=YHU0B56401R&log%24=prottop&blast_rank=1&list_uids=72161024