Coding

Part:BBa_M11010:Design

Designed by: Joseph Camire   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-18)

Thioredoxin Peroxidase II, Cricetulus griseus, mRNA Coding


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 474
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene codes for an mRNA of a reported eukaryotic arsenic binding protein. This gene, as submitted, is the mRNA sequence without modifications from Cricetulus griseus. There is an SpeI restriction site still present in the sequence that would need to be mutated to allow use of the gene in the standard IGEM Biobrick construction.

Source

LOCUS AF221841 600 bp mRNA linear ROD 10-APR-2003 DEFINITION Cricetulus griseus thioredoxin peroxidase II mRNA, complete cds. ACCESSION AF221841 VERSION AF221841.1 GI:6942232 SOURCE Cricetulus griseus (Chinese hamster)

References

Chang, K.N.:Lee, T.C.; Tam, M.F.; Chen, Y.C.; Lee, L.W.; Lee S.Y.: Lin, P.J.; Huang, R.N., Identification of galectin I and thioredoxin peroxidase II as two arsenic-binding proteins in Chinese Hamster Ovary cells. Biochem J, 2003, 371, (Pt 2), 495-503.