Part:BBa_M11008:Design

NADH-dependent butanol dehydrogenase B (bdhB/BDH II)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1030
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1030
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1030
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

bdhB comes from the (-) strand, bdhB precedes bdhA in the actual sequence from Clostridium acetobutylicum ATCC 824

Source

Clostridium acetobutylicum ATCC 824: NC_003030 (3940880bp)

References

[1] "Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes." Walter K.A., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 174:7149-7158(1992) [PubMed: 1385386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

[2] "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

[3] "Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum." Petersen D.J., Welch R.W., Walter K.A., Mermelstein L.D., Papoutsakis E.T., Rudolph F.B., Bennett G.N. Ann. N. Y. Acad. Sci. 646:94-98(1991) [PubMed: 1809209] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.